About Sony Biotechnology

  • Enabling flow cytometry analysis of tissues with spectral flow cytometry

    23 Jun 2017

    Flow cytometry has long been considered a tool of hematologists and immunologists who primarily work with the hematopoetic system.  Flow cytometry is capable of performing the simultaneous detection of more than 20 parameters; however it requires the sample to be prepared into a single cell suspension. The need for a single cell suspension makes the sample preparation more intensive for cells derived from tissues. Many tissues are also highly autofluorescent, which results in background noise that limits detection of low levels of expression on conventional flow cytometers.

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  • Learning more about CRISPR from experts

    23 Jun 2017

    Listen to Dr. Feng Zhang who was first to adapt CRISPR-Cas9 for genome editing on eukaryotic cells explain CRISPR:

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  • Sorting iPSC derived Cardiomyocytes using the SH800 Cell Sorter

    15 Jun 2017

    Pluripotent stem cells (PSCs) offer an unprecedented opportunity for both disease modeling and personalized medicine. In particular, PSC derived cardiomyocytes (CM) mature into adult cardiomyocytes when transplanted into neonatal rat hearts allowing iPSC modeling of cardiomyopathy. A recent published study by Kwong et al 1 shows the successful isolation of cardiac progenitor cells (CPCs) from mouse embryonic stem cells. To achieve this, a mESC line expressing Isl1-Cre; Rosa-RFP; aMHC-GFP was generated. The expression of Rosa-RFP allowed tracking of CPCs destined to mature into CM. RFP+ CPCs were sorted using the Sony SH800 cell sorter using the 130um microfluidics sorting chip.

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  • Using flow cytometry to evaluate compatibility of tyrosine kinase inhibitors with immunotherapy

    05 Jun 2017

    Immunotherapy has shown great promise as a cancer treatment resulting in several FDA approved drugs and clinical trials. For example, a promising therapy in several tumors is the blocking of the PD-1/PD-L1 interaction that is over expressed on many types of tumor cells.  However, success depends on the patient’s immune system to respond to the tumor.

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  • Standardization In - Variability Out – Reliable Data from your Cell Analyzer

    25 May 2017

    As most researchers know, all instruments have some variability and over time settings continue to drift. Obviously, instrument variability is “bad” because it can unwittingly impact your data – forcing you to re-run experiments to determine what caused data anomalies.

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  • Using cell sorting to isolate cells with genes edited by CRISPR-Cas9

    19 May 2017

    With the ability to easily identify and isolate single cells from a heterogeneous phenotypic population, a cell sorter is a useful tool for laboratories studying and applying the CRISPR technology as a tool for editing genes and creating single cell libraries.

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  • How the immune system impacts liver regeneration

    18 May 2017

    Unlike most organs in the human body the liver is able to regenerate allowing donors and patients to re-grow resected tissue. The success and rate of regeneration can be negatively impacted by several factors including sub-septic conditions. One factor is LFA-1, an integrin lymphocyte function-associated antigen-1.

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  • Simple Panels for the Study of T-Cells

    01 May 2017

    T-cells are part of the adaptive immune system with several subtypes including CD8+ effector T-cells and CD4+ helper T-cells. Main categories can be divided into several sub-categories. What are the critical markers to study T-cells? From the key subsets how can other subsets be defined?

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  • Explain CRISPR-Cas-9 to your Mom

    01 May 2017

    Thank your super hero mom this Mother’s Day by giving her some quality time and something to remember—the gift of knowing about the work you do that makes the world a better place. To help you with what could be the best little talk ever here’s a 5-minute animation from the Royal Society that explains gene editing and the importance CRISPR-Cas-9 we hope you will find useful. Happy Mother’s Day to all, thank you for what you do. Video: What is gene editing and how does it work?

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  • Selecting the Best Clone for Your Flow Cytometry Experiments

    03 Apr 2017

    With literally thousands of commercially available fluorochrome conjugated antibodies for flow cytometry, there are many choices for your application. In many cases several clones are available to the same target. How do you evaluate which clones are best for your research?

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