About Sony Biotechnology

  • Detection of in vivo cell movement by flow cytometry

    16 Oct 2017

    Researchers used spectral flow cytometry and fluorescent proteins to capture both movement and interactions of immune cells in live mice.

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  • Obtaining single cell suspensions of brain tissues

    29 Sep 2017

    Recent advances in genome editing and the application of fluorescent proteins have accelerated Interest in isolating specific populations of brain cells from mixed populations. Researchers are also using cell sorting to isolate single cells for expansion and analysis.

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  • Gaining an edge – taking advantage of novel fluorescent proteins with Flow Cytometry

    18 Sep 2017

    Placing several fluorescent proteins together in a flow cytometry panel offers greater power and capability for experiments. However, handling autofluorescent signal with fluorescent proteins is out of reach for conventional flow cytometetry users. Sony spectral flow cytometry analyzers enable researchers to harness up to five near infra-red fluorescent proteins in a single experiment. Moreover, spectral technology lets users accurately identify autofluorescence, and eliminate it if needed.

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  • What do olives and cell sorting have in common?

    23 Aug 2017

    Researchers are isolating the Candidatus Erwinia dacicola bacteria from the guts of olive flies on a Sony SH800S and amplifiying Genomic DNA to construct a library for sequencing.

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  • Five ways to save money on antibodies for flow cytometry

    11 Aug 2017

    Learn tips from the field for managing the costs of antibodies for flow cytometry.

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  • Four reasons to put bacteria into your cell sorter

    27 Jul 2017

    Bacteria such as E. coli are popular model systems for engineering and production of modified proteins. Yet the idea of putting bacteria in a cell sorter conjures unwelcome images. Take heart, the Sony SH800 cell sorter, simplifies decontamination by quickly and easily letting researchers replace key components that come in contact with the sample. Here are some examples, and publication citations.

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  • Learning more about CRIPSR from the experts – Dr. Jennifer Doudna

    25 Jul 2017

    Listen to Dr. Jennifer Doudna, one of the discoverers of CRISPR systems discusses how the new genome engineering technology was discovered.

    Video: Genome Engineering with CRISPR-Cas9: Birth of a Breakthrough Technology

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  • Will small amounts of preservative kill my cells?

    11 Jul 2017

    Most commercially available antibodies contain small amounts of preservatives such as sodium azide to prevent microbial growth. However, sodium azide is also toxic to mammalian cells as it inhibits cellular respiration. Actual toxicity varies by cell type with neuronal cells being most sensitive. Toxicity is concentration, time, and temperature dependent. For most cell sorting experiments the health of cells are not impacted because the antibody is diluted and cells are typically incubated on ice for less than one hour.

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  • Learn About Spectral Flow and DeNovo FCS Express 6

    06 Jul 2017

    Join us for a free webinar on Spectral Flow and FCS Express 6 which provides native support for Sony spectral data files. See how spectral flow cytometry delivers better data and simplifies panel design. In addition we’ll show how seamless integration between FCS Express and Sony spectral flow cytometry analyzers allows you to move quickly from acquisition to expanded data visualization with spectral overlays, tSNE, Spade, and plate based heat maps.

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  • Enabling flow cytometry analysis of tissues with spectral flow cytometry

    23 Jun 2017

    Flow cytometry has long been considered a tool of hematologists and immunologists who primarily work with the hematopoetic system.  Flow cytometry is capable of performing the simultaneous detection of more than 20 parameters; however it requires the sample to be prepared into a single cell suspension. The need for a single cell suspension makes the sample preparation more intensive for cells derived from tissues. Many tissues are also highly autofluorescent, which results in background noise that limits detection of low levels of expression on conventional flow cytometers.

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