KIRAVIA Dyes™

KIRAVIA Dyes

The KIRAVIA Dyes™ are organic polymers with fluorescent dyes loaded precisely on a novel backbone developed by Sony, based on proprietary technology. This family of fluorescent dyes can be used on both spectral and conventional flow cytometers. Designed for ease of use, KIRAVIA Dyes do not require a special buffer system or sample preparation protocol.


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Sony is offering free shipping on antibody and ancillary flow cytometry reagents until September 30, 2020.* Simply add reagents to your online shopping cart and automatically receive free shipping at check-out.

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Features

  • Novel backbone technology to enable development of dyes with improved properties
  • Ready for use in a wide range of life science applications

To further life science research in the regenerative medicine and immunology fields, Sony has released a portfolio of dyes conjugated to antibodies, giving researchers new choices for panel design. The conjugates can be used in a variety of multicolor applications.

KIRAVIA Blue 520™
The first dye in the family, KIRAVIA Blue 520™ (KB520), is excited by the 488-nm laser, with a maximum signal at 520 nm. Recommended for experiments on flow cytometers equipped with a 488-nm laser, this novel dye is ideal for panels with dim expressors. Its excellent brightness provides better discrimination and resolution. KB520 can replace fluorochromes that use the 530/30 (FITC) detector. In high parameter experiments KB520 can be used to detect antigens with a variety of abundance levels, but would ideally be saved for an antigen with ubiquitous and/or variable expression levels in the sample. The KB520 conjugated antibodies exhibit higher stability and brightness compared to FITC conjugates.

Exmax Emmax Filter Relative Brightness
497 nm 520 nm 520/30 ☑ ☑ ☑ ☑

Figure 1 Graphic

Figure 1.

The figure shows a cell with surface antigens to which the antibodies can bind. The structure of the KIRAVIA Dyes allow for the fluorochromes to be precisely positioned for increased brightness.


Figure 2 Graphic

Figure 2. KIRAVIA Blue 520 Excitation and Emission

Sony KB520 fluorochrome is a blue laser excitable dye with a 497 nm excitation max and 520 nm emission max.


Figure 3 Graphic

Figure 3. KIRAVIA Blue 520 Emission

KIRAVIA Blue 520 emission spectra on the SA3800 Spectral Analyzer.


Figure 4 Graphic

Figure 4.

Comparison of KB520 (green) with Alexa Fluor® 488 (blue) using the ID7000™ Spectral Cell Analyzer. The KB520 dye is brighter than Alexa Fluor® 488.


Figure 5 Graphic

Figure 5. CD3 Positive and Negative Cell Populations

Analysis of Human PBMCs CD3 KB520 populations using the ID7000 Spectral Cell Analyzer.


Figure 6 Graphic

Figure 6.

Human PBMCs were stained with CD3 KB520 (blue) and CD3 Alexa Fluor® 488 (red) and compared vs CD8 Brilliant Violet 421 (BV421) on the BD LSR Fortessa™ analyzer.


*Free shipping reagent offer valid in the United States and Europe through September 30, 2020. Orders must be placed on sonybiotechnology.com. Not valid for distributors. Cannot be combined with other offers. Excludes ClearFlow™ Sheath Fluid, ClearSort™ Sheath Fluid and Instrument QC bead products.

ID7000, and KIRAVIA are trademarks of Sony Corporation. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. BD LSRFortessa is a trademark of Becton, Dickinson and Company. The SA3800 and ID7000 analyzers are classified as Class 1 Laser Products. For Research Use Only. Not for use in diagnostic or therapeutic procedures.