Table 1. MA900 instrument configuration for a 12-color immunophenotyping panel The MA900 from Sony meets the demands of most sorting applications, supporting up to 4 lasers, 12 fluorescence parameters, and 4-way sorting. Powerful, modern technologies built into the MA900 system dramatically simplify operation to make sorting less subjective and improve reliability. For this application note, the MA900 cell sorter was used for analysis and sorting of a 12-color immunophenotyping panel (Table 1). Human peripheral blood mononuclear cells (PBMCs) were stained with reagents for analysis of common T-cell, B-cell, NK-cell, and monocyte subsets. These subsets were identified using different gating strategies, and sort performance was evaluated by performing 4-way sorting of T cells, NK cells, B cells, and monocytes, followed by post-sort analysis.   Methods Whole blood was lysed with RBC Lysis Buffer and stained with antibodies against Alexa Fluor® 488 CD3, PerCP-Cy™5.5 CD4, Brilliant Violet 570™ CD8, APC-Cy™7 CD14, PE/Dazzle™ CD16, Brilliant Violet 421™ CD19, Brilliant Violet 605™ CD20, Brilliant Violet 510™ CD27, APC CD45RA, PE-Cy7 CD56, Alexa Fluor® 700 HLA-DR, and PE TCR gamma/delta. All reagents were from Sony Biotechnology Inc. Cells were incubated with the antibodies for 20 minutes on ice, washed twice with staining buffer, and...

Join this free webinar to learn how to optimize sample preparation and obtain quality data from a multiplexed, single-cell sequencing assay. Increasingly, single-cell sequencing is used to profile the transcriptome of rare cell populations, resolve antigen binding specificities of T cells, and trace distinct cell lineages in development.

Single-cell sequencing has proven invaluable to understanding the biology of heterogeneous samples such as brain or tumor tissues. However, sorting individual cells into wells for downstream sequencing does not provide adequate throughput needed for meaningful analysis of the epigenetic states of the cells. To address this challenge, this research team developed a new methodology to analyze properties of single cells by labeling cell nuclei with DNA "barcodes" and distributing them using the SH800 sorter from Sony Biotechnology.

Can antibodies be used as potential complements to vaccines? This team scours the planet looking for human donors to isolate PBMCs from those exposed to Rift Valley Fever in Kenya, to Ebola survivors. Supporting this important work, the Sony SH800 cell sorter has become an essential tool to isolate the right cells for a pipeline to generate antibody sequences that will ultimately build effective medical countermeasures.

The MA900 Multi-Application Cell Sorter offers an affordable option to labs that need flexible detection capability with its free-form PMT layout, allowing users to set up a variety of panels without time-consuming changes in the optics.

Learn how scientists have built a scalable approach using CRISPR-Cas9 and the SH800 cell sorter from Sony to research restoring vision in people with optic neuropathies.

Learn how the automated cleaning function of the MA900 Multi-Application Cell Sorter saves time and reduces errors for multi-user labs.

David Savage's laboratory uses progressive cell sorting of large libraries to allosterically engineer protein biosensors and direct the genome editing activity of Cas9. Second in a series of real-world applications for the Sony SH800 cell sorter.

A study of porcine iPS cells in an SLA-matched setting is a possible robust model for transplantation of human iPS cells in an HLA-matched setting. The Sony SH800 was used to perform the NK mediated cytotoxicity assay.

Join flow cytometry experts for a webinar on best practices in cell sorting. This webinar will be valuable to those with any level of cell sorting experience.