- Sony Biotechnology
- Sony Biotechnology
Learn how the automated cleaning function of the MA900 Multi-Application Cell Sorter saves time and reduces errors for multi-user labs.
David Savage's laboratory uses progressive cell sorting of large libraries to allosterically engineer protein biosensors and direct the genome editing activity of Cas9. Second in a series of real-world applications for the Sony SH800 cell sorter.
A study of porcine iPS cells in an SLA-matched setting is a possible robust model for transplantation of human iPS cells in an HLA-matched setting. The Sony SH800 was used to perform the NK mediated cytotoxicity assay.
Learn how a developmental cell biology lab combined single-cell sorting with RNA sequencing to accelerate stem cell maturation and model cardiac abnormalities. This is the first in our profile series of real-world applications for the Sony SH800 cell sorter.
Learn how the Sony SH800S cell sorter supports gene expression profiling to purify phenotypically similar cell populations in bulk.
Researchers used spectral flow cytometry and fluorescent proteins to capture both movement and interactions of immune cells in live mice.
Placing several fluorescent proteins together in a flow cytometry panel offers greater power and capability for experiments. However, handling autofluorescent signal with fluorescent proteins is out of reach for conventional flow cytometetry users. Sony spectral flow cytometry analyzers enable researchers to harness up to five near infra-red fluorescent proteins in a single experiment. Moreover, spectral technology lets users accurately identify autofluorescence, and eliminate it if needed.
Learn tips from the field for managing the costs of antibodies for flow cytometry.
Bacteria such as E. coli are popular model systems for engineering and production of modified proteins. Yet the idea of putting bacteria in a cell sorter conjures unwelcome images. Take heart, the Sony SH800 cell sorter, simplifies decontamination by quickly and easily letting researchers replace key components that come in contact with the sample. Here are some examples, and publication citations.
Most commercially available antibodies contain small amounts of preservatives such as sodium azide to prevent microbial growth. However, sodium azide is also toxic to mammalian cells as it inhibits cellular respiration. Actual toxicity varies by cell type with neuronal cells being most sensitive. Toxicity is concentration, time, and temperature dependent. For most cell sorting experiments the health of cells are not impacted because the antibody is diluted and cells are typically incubated on ice for less than one hour.