Learn how scientists have built a scalable approach using CRISPR-Cas9 and the SH800 cell sorter from Sony to research restoring vision in people with optic neuropathies.

A study of porcine iPS cells in an SLA-matched setting is a possible robust model for transplantation of human iPS cells in an HLA-matched setting. The Sony SH800 was used to perform the NK mediated cytotoxicity assay.

Learn how the Sony SH800S cell sorter supports gene expression profiling to purify phenotypically similar cell populations in bulk.

Researchers used spectral flow cytometry and fluorescent proteins to capture both movement and interactions of immune cells in live mice.

Placing several fluorescent proteins together in a flow cytometry panel offers greater power and capability for experiments. However, handling autofluorescent signal with fluorescent proteins is out of reach for conventional flow cytometetry users. Sony spectral flow cytometry analyzers enable researchers to harness up to five near infra-red fluorescent proteins in a single experiment. Moreover, spectral technology lets users accurately identify autofluorescence, and eliminate it if needed.

Researchers are isolating the Candidatus Erwinia dacicola bacteria from the guts of olive flies on a Sony SH800S and amplifiying Genomic DNA to construct a library for sequencing.

Join us for a free webinar on Spectral Flow and FCS Express 6 which provides native support for Sony spectral data files. See how spectral flow cytometry delivers better data and simplifies panel design. In addition we’ll show how seamless integration between FCS Express and Sony spectral flow cytometry analyzers allows you to move quickly from acquisition to expanded data visualization with spectral overlays, tSNE, Spade, and plate based heat maps.

Listen to Dr. Feng Zhang who was first to adapt CRISPR-Cas9 for genome editing on eukaryotic cells explain CRISPR:

Pluripotent stem cells (PSCs) offer an unprecedented opportunity for both disease modeling and personalized medicine. In particular, PSC derived cardiomyocytes (CM) mature into adult cardiomyocytes when transplanted into neonatal rat hearts allowing iPSC modeling of cardiomyopathy. A recent published study by Kwong et al 1 shows the successful isolation of cardiac progenitor cells (CPCs) from mouse embryonic stem cells. To achieve this, a mESC line expressing Isl1-Cre; Rosa-RFP; aMHC-GFP was generated. The expression of Rosa-RFP allowed tracking of CPCs destined to mature into CM. RFP+ CPCs were sorted using the Sony SH800 cell sorter using the 130um microfluidics sorting chip.

Immunotherapy has shown great promise as a cancer treatment resulting in several FDA approved drugs and clinical trials. For example, a promising therapy in several tumors is the blocking of the PD-1/PD-L1 interaction that is over expressed on many types of tumor cells.  However, success depends on the patient’s immune system to respond to the tumor.