About Sony Biotechnology

  • Standardization In - Variability Out – Reliable Data from your Cell Analyzer

    As most researchers know, all instruments have some variability and over time settings continue to drift. Obviously, instrument variability is “bad” because it can unwittingly impact your data – forcing you to re-run experiments to determine what caused data anomalies.

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  • Using cell sorting to isolate cells with genes edited by CRISPR-Cas9

    With the ability to easily identify and isolate single cells from a heterogeneous phenotypic population, a cell sorter is a useful tool for laboratories studying and applying the CRISPR technology as a tool for editing genes and creating single cell libraries. CRISPR genome editing tools can be used to remove, change or insert DNA sequences into genes. The resulting mutations are maintained in the genome as cells proliferate –in contrast to transient transfection methods. Edits to genes are accomplished by directing the Cas9 nuclease to cleave DNA at a specific sequence and a guide RNA (gRNA) into the cell through transfection (or electroporation). For the edit to be completed the cell must be successfully transfected and the DNA cleaved by the Cas9 (referred to as cleavage efficiency). Many factors can impact cleavage efficiency including the efficiency of the transfection. A commonly used method to identify cells that have been successfully transfected is to include a plasmid encoding for a fluorescent protein such as eGFP in the transfection along with Cas9 and gRNA. Cells can then be sorted based on the expression of the fluorescent protein. The SH800 sorter, lets researchers deposit target cells into 96- or 384 multi-well devices and...

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  • How the immune system impacts liver regeneration

    Unlike most organs in the human body the liver is able to regenerate allowing donors and patients to re-grow resected tissue. The success and rate of regeneration can be negatively impacted by several factors including sub-septic conditions. One factor is LFA-1, an integrin lymphocyte function-associated antigen-1.

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  • Simple Panels for the Study of T-Cells

    T-cells are part of the adaptive immune system with several subtypes including CD8+ effector T-cells and CD4+ helper T-cells. Main categories can be divided into several sub-categories. What are the critical markers to study T-cells? From the key subsets how can other subsets be defined?

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  • Explain CRISPR-Cas-9 to your Mom

    Thank your super hero mom this Mother’s Day by giving her some quality time and something to remember—the gift of knowing about the work you do that makes the world a better place. To help you with what could be the best little talk ever here’s a 5-minute animation from the Royal Society that explains gene editing and the importance CRISPR-Cas-9 we hope you will find useful. Happy Mother’s Day to all, thank you for what you do. Video: What is gene editing and how does it work?

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  • Selecting the Best Clone for Your Flow Cytometry Experiments

    Tips for choosing the best clone for flow cytometry experiments to improve research quality and save time and money.

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  • Make the Connection: Cell Sorting to Separate Brain Cells

    Effective techniques for sorting brain cells learned from recent successul studies.

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  • Cystic Fibrosis Foundation Therapeutics in Boston BizJournal

    The Cystic Fibrosis Foundation Therapeutics Inc. has opened a new laboratory in Lexington, MA. and will be using the Sony SH800 for cell sorting. The lab will focus on the discovery of promising drugs for both common and rare mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene.

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