- Sony Biotechnology
- Sony Biotechnology
Available On Demand—Originally presented on Thursday, March 14, 2019 by Dr. Peter Lopez and Dr. Rui Gardner This SelectScience® webinar will be valuable to those with any level of cell sorting experience who use flow cytometry and want to improve their experimental outcomes. This session addresses three key considerations for effective cell sorting. You’ll learn: How purity, efficiency, and speed influence good sorting outcomes—and choices you’ll need to make to optimize sorting for your application. How instrument characteristics can influence cell sorting performance—and how to select what will work best for your experiment. How to set up for plate sorting to improve single cell deposition efficiency View On Demand →
The Sony MA900 Multi-Application Cell Sorter has been nominated for the Best New Life Sciences Product of 2018! Scientists worldwide are voting in this year's Scientists' Choice Awards® for the best product, and we are thrilled that the MA900 is being recognized as a game changer in the industry. The MA900 is packed with innovative, modern capabilities including 12 fluorescence parameters and 4-way sorting, providing choice and flexibility for most flow cytometry tests and applications. Advanced automation that makes sorting less subjective and more reliable. Sensors and software to dramatically simplify aseptic cleaning, QC, sorting, and troubleshooting. Intelligent and adaptable to meet your needs. Choose from eight default and five custom sort modes to achieve desired purity and yield. Easy to learn and use. Easy-to-use wizards guide users through workflows from start to finish, so researchers can focus more time on science. ✔️ Please click here to vote for the MA900 today!
Much of cell research happens in the context of cultured or modified cells. More biologically relevant experiments can be done with blood. However, blood cannot entirely capture what takes place within a live organism. In their paper Futamura, et al. describes a method to study the movement and subsequent interactions of immune cells in live mice by flow cytometry.
Recent advances in genome editing and the application of fluorescent proteins have accelerated Interest in isolating specific populations of brain cells from mixed populations. Researchers are also using cell sorting to isolate single cells for expansion and analysis.
Bacteria, most notably the gut microbiome, have gained increased attention for their role in agriculture and human disease. However study of individual bacterium can be challenging since most bacteria do not grow under simple culture conditions. In a recent study, Blow, et al. have published a draft sequence of the bacteria, Candidatus Erwinia dacicola (Enterobacteriaceae). This bacteria has a symbiotic relationship with the Bactrocera oleae, also known as the olive fly. The larva of the olive fly grow in unripened olives leading to reduced crop yields. The larva has adapted to the special environment of the unripened olive with help from Candidatus Erwinia dacicola bacteria. Without bacteria the larva do not develop.
1. Purchase larger sizes of common antibodies on established fluorochromes. Packaging and shipping antibodies can be costly. Therefore smaller sizes typically cost greater than two times more compared to larger sizes.
Listen to Dr. Jennifer Doudna, one of the discoverers of CRISPR systems discusses how the new genome engineering technology was discovered.
With the ability to easily identify and isolate single cells from a heterogeneous phenotypic population, a cell sorter is a useful tool for laboratories studying and applying the CRISPR technology as a tool for editing genes and creating single cell libraries.
With literally thousands of commercially available fluorochrome conjugated antibodies for flow cytometry, there are many choices for your application. In many cases several clones are available to the same target. How do you evaluate which clones are best for your research?
Cell sorting is a valuable technique for isolating cells from mixed populations. This technique can be challenging when working with tissues such as brain, as it requires single cell suspensions. With recent advances in genome editing and fluorescent protein applications, the interest in sorting brain cells has been increasing. Several studies have been published in which scientists have successfully isolated stem cells from whole brain homogenate using cell sorting.
Guidelines for sorting brain cells.