- Sony Biotechnology
- Sony Biotechnology
Much of cell research happens in the context of cultured or modified cells. More biologically relevant experiments can be done with blood. However, blood cannot entirely capture what takes place within a live organism. In their paper Futamura, et al. describes a method to study the movement and subsequent interactions of immune cells in live mice by flow cytometry.
Recent advances in genome editing and the application of fluorescent proteins have accelerated Interest in isolating specific populations of brain cells from mixed populations. Researchers are also using cell sorting to isolate single cells for expansion and analysis.
Bacteria, most notably the gut microbiome, have gained increased attention for their role in agriculture and human disease. However study of individual bacterium can be challenging since most bacteria do not grow under simple culture conditions. In a recent study, Blow, et al. have published a draft sequence of the bacteria, Candidatus Erwinia dacicola (Enterobacteriaceae). This bacteria has a symbiotic relationship with the Bactrocera oleae, also known as the olive fly. The larva of the olive fly grow in unripened olives leading to reduced crop yields. The larva has adapted to the special environment of the unripened olive with help from Candidatus Erwinia dacicola bacteria. Without bacteria the larva do not develop.
1. Purchase larger sizes of common antibodies on established fluorochromes. Packaging and shipping antibodies can be costly. Therefore smaller sizes typically cost greater than two times more compared to larger sizes.
Listen to Dr. Jennifer Doudna, one of the discoverers of CRISPR systems discusses how the new genome engineering technology was discovered.
With the ability to easily identify and isolate single cells from a heterogeneous phenotypic population, a cell sorter is a useful tool for laboratories studying and applying the CRISPR technology as a tool for editing genes and creating single cell libraries.
With literally thousands of commercially available fluorochrome conjugated antibodies for flow cytometry, there are many choices for your application. In many cases several clones are available to the same target. How do you evaluate which clones are best for your research?
Cell sorting is a valuable technique for isolating cells from mixed populations. This technique can be challenging when working with tissues such as brain, as it requires single cell suspensions. With recent advances in genome editing and fluorescent protein applications, the interest in sorting brain cells has been increasing. Several studies have been published in which scientists have successfully isolated stem cells from whole brain homogenate using cell sorting.
Guidelines for sorting brain cells.
To identify and test new treatments for cystic fibrosis, Cystic Fibrosis Foundation Therapeutics Inc. has opened a new laboratory in Lexington, MA. The team of 25 will focus on the discovery of promising drugs for both common and rare mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene. For cell sorting the lab selected the Sony SH800 (placed inside a biosafety cabinet).