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- Sony Biotechnology
- Sony Biotechnology
CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
1. Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed