Marker |
Clone |
Function |
Violet Laser |
Blue Laser |
Red Laser |
CD1d |
51.1 |
Downregulated upon B-cell activation |
BV510
(2351565, 2351570) |
PerCP/Cy5.5
(2351555, 2351560)PE
(2351525, 2351530)PE/Cy7 2351545, 2351550) |
APC
(2351535, 2351540) |
CD5 |
L17F12 |
Differentiation |
None |
PerCP/Cy5.5 (2420025, 2420030), PE/Cy7 (2420035, 2420040) |
APC/Cy7 (2420045, 2420050) |
CD5 |
UCHT2 |
Differentiation |
PacBlue
(2103115, 2103120)BV421
(2103125, 2103130) |
FITC
(2103025, 2103030)PerCP
(2103085, 2103090)PerCP/Cy5.5
(2103095, 2103100)
PE
(2103035, 2103040)
PE/Cy7
(2103105, 2103110) |
APC
(2103055, 2103060)AF647
(2103080) |
CD10 |
HI10a |
Expressed prior to DJ rearrangement |
BV421
(2161085, 2161090)BV510
(2161095, 2161100)BV605
(2161105, 2161110) |
FITC
(2161035, 2161040)PerCP/Cy5.5
(2161075, 2161080)PE
(2161015, 2161020)
PE/Cy5
(2161030)
PE/Cy7
(2161065, 2161070) |
APC
(2161045, 2161050)APC/Cy7
(2161060) |
CD20
(cytoplasmic) |
1412 |
Useful for intracellular staining of CD20 |
|
AF488
(2302520)PerCP/Cy5.5
(2302540)PE
(2302550) |
AF647
(2302530) |
CD21 |
Bu32 |
Important for complement binding |
|
FITC
(2374545, 2374550)PerCP/Cy5.5
(2374535, 2374540)PE
(2374515, 2374520)
PE/Cy7
(2374555, 2374560) |
APC
(2374525, 2374530) |
CD23 |
EBVCS-5 |
Regulates the production of IgE |
BV421
(2292605, 2292610) |
FITC
(2292525, 2292530)PerCP/Cy5.5
(2292585, 2292590)PE
(2292535, 2292540)
PE/Cy7
(2292580) |
APC
(2292565, 2292570)APC/Cy7
(2292600) |
CD24 |
ML5 |
Decreased expression with B cell activation; not expressed on plasma cells |
BV5421
(2155605, 2155610)BV510
(2155625, 2155630)BV605
(2155615, 2155620) |
FITC
(2155515, 2155520)AF488
(2155540)PerCP
(2155565, 2155570)
PerCP/Cy5.5
(2155575, 2155580)
PE
(2155525, 2155530)
PE/Cy7 2155595, 2155600) |
APC
(2155585, 2155590)AF647
(2155545, 2155550) |
CD27 |
LG.3A10 |
Memory and activation marker |
PacBlue
(1221085, 1221090) |
PerCP/Cy5.5
(1221065, 1221070)PE/Cy7
(1221075, 1221080) |
APC
(1221055, 1221060) |
|
M-T271 |
|
PacBlue
(2382065, 2382070)BV421
(2382085, 2382090)BV510
(2382095, 2382100) |
FITC
(2382015, 2382020)PerCP/Cy5.5
(2382035, 2382040)PE
(2382025, 2382030)
PE Dazzle 594
(2382105, 2382110)
PE/Cy7
(2382055, 2382060) |
APC
(2382045, 2382050)AF700
(2382075, 2382080) |
|
O323 |
|
PacBlue
(2114105, 2114110)BV421
(2114115, 2114120)BV510
(2114175, 2114180)
BV570
(2114125)
BV605
(2114145, 2114150)
BV650
(2114135, 2114140)
BV711
(2114165, 2114170)
BV785
(2114155, 2114160) |
FITC
(2114025, 2114030)PerCP
(2114085, 2114090) PerCP/Cy5.5
(2114095, 2114100)PE
(2114035, 2114040)
PE/Cy7
(2114185, 2114190 |
APC
(2114045, 2114050)AF647
(2114060)AF700
(2114065, 2114070)
APC/Cy7
(2114075, 2114080) |
CD48 |
BJ40 |
Highly expressed on all B cell subsets |
PacBlue
(2283510) |
FITC
(2283525, 2283530)PE
(2283535, 2283540) |
|
CD70 |
113-16 |
Expressed on activated B cells; involved in expansion and differentiation |
|
FITC
(2375525, 2375530)PerCP/Cy 5.5
(2375535, 2375540)PE
(2375515, 2375520) |
APC
(2375545, 2375550) |
CD79a |
HM47 |
Important for B cell development and function |
|
PerCP/Cy 5.5
(2267540)PE
(2267515, 2267520)PE/Cy7
(2267545, 2267450) |
APC
(2267525, 2267530) |
CD80 |
2D10 |
Expressed on germinal center cells |
BV421
(2126105, 2126110)BV605
(2126125)BV650
(2126135) |
FITC
(2126025, 2126030)AF488
(2126065, 2126070)PE
(2126035, 2126040)
PE/Cy5
(2126045, 2126050)
PE/Cy7
(2126085, 2126090) |
|
CD81
(TAPA-1) |
5A6 |
Important for B-cell signaling |
|
FITC
(2347515, 2347520)PerCP/Cy5.5
(2347535, 2347540)PE
(2347525, 2347530)
PE/Cy7
(2347555, 2347560) |
APC
(2347545, 2347550) |
CD84 |
CD84.1.21 |
Highly expressed on all B cell subsets |
|
PE
(2230035, 2230040) |
|
CD93 |
VIMD2 |
B-lineage precursor molecule |
|
PE
(2280535, 2280540) |
|
CD126 |
UV4 |
Expressed on plasmablasts and plasma cells |
|
PerCP/Cy5.5
(2364055, 2364060)PE
(2364015, 2364020)PE/Cy7
(2364045, 2364050) |
APC
(2364025, 2364030) |
CD138
(Syndecan-1) |
DL-101 |
Plasma cell marker |
|
FITC
(2361515, 2361520)PerCP/Cy5.5
(2361545, 2361550)PE
(2361525, 2361530) |
APC
(2361535, 2361540) |
|
Ml15 |
|
BV421
(2382575, 2382580)BV510
(2382585, 2382590)BV605
(2382595, 2382600)
BV711
(2382605, 2382610) |
FITC
(2382535, 2382540)PerCP/Cy5.5
( 2382545, 2382550)PE
(2382515, 2382520)
PE/Cy7
(2382565, 2382570) |
APC
(2382525, 2382530)AF647
(2382615, 2382620)AF700
(2382555, 2382560) |
CD150
(SLAM) |
A12
(7D4) |
Expressed on B cell subsets |
|
FITC
(2131530)AF488
(2131560)PE
(2131535, 2131540) |
|
CD156c
(ADAM10) |
SHM14 |
May be involved in regulation of IgE |
|
PE
(2363515, 2363520) |
APC
(2363525, 2363530) |
CD170
(Siglec-5) |
1A5 |
Expressed on a subset of mature naïve and pre-germinal center cells |
|
PE
(2360015, 2360020) |
APC
(2360025, 2360030) |
CD184
(CXCR4) |
12G5 |
Expressed on all B cells with highest amounts on germinal center B cells |
BV421
(2132585, 2132590)BV605
(2132605, 2132610) |
PerCP/Cy5.5
(2132575, 2132580)PE
(2132525, 2132530)PE Dazzle 594
(2132625, 2132630)
PE/Cy5
(2132535, 2132540)
PE/Cy7
(2132565, 2132570) |
APC
(2132545, 2132550) |
CD185
(CXCR5) |
J252D4 |
Expressed on all B cells |
PacBlue
(2384585, 2384590)BV5421
(2384595, 2384600)BV510
(2384605, 2384610) |
FITC
(2384565, 2384570)AF488
(2384555, 2384560)PerCP/Cy5.5
(2384545, 2384550)
PE
(2384515, 2384520)
PE Dazzle 594
(2384635, 2384640)
PE/Cy7
(2384615, 2384620) |
APC
( 2384535, 2384540)AF647
(2384525, 2384530)AF700
(2384575, 2384580)
APC/Cy7
(2384625, 2384630) |
CD196
(CCR6) |
GO34E3 |
Expressed on pre-germinal center B cells |
BV521
(2367035, 2367040)BV510
(2367115)BV605
(2367095, 2367100)
BV650
(2367125, 2367130)
BV785
(2367105, 2367110) |
FITC
(2367055, 2367060)AF488
(2367065, 2367070)PerCP/Cy5.5
(2367025, 2367030)
PE
(2367045, 2367050)
PE Dazzle 594
(2367145, 2367150)
PE/Cy7
(2367085, 2367090) |
APC
(2367075, 2367080)AF647
(2367015, 2367020) |
CD252
(OX40L, TNFSF4) |
11C3.1 |
Expressed at all stages of B-cell development |
|
PE
(2231535, 2231540) |
|
CD255
(TWEAK) |
CARL-1 |
Differentially expressed on B-cell subsets |
|
PE
(2141525) |
|
CD267
(TACI, TNFRSF13B) |
1A1 |
Has a negative regulatory role in B cell homeostasis |
|
PE
(2159530)PE Dazzle 594
(2159545, 2159550) |
|
CD268
(BAFF-R, TNFR) |
11C1 |
Important for B cell development and survival |
|
FITC
(2184520)PerCP/Cy5.5
(2184585, 2184590)PE
(2184525, 2184530)
PE/Cy7
(2184595, 2184600) |
APC
(2184580)AF647
(2184570)APC/Cy7
(2184560) |
CD269
(BCMA, TNFRSF17) |
19F2 |
Important for plasma cell survival. Upregulated upon activation. |
|
PE
(2387515, 2387520) |
APC
(2387525, 2387530) |
CD270
(HVEM, TR2) |
122 |
Differentially expressed on B cell subsets |
|
PerCP/Cy5.5
(2194055, 2194060)PE
(2194025, 2194030)PE/Cy7
(2194045, 2194050) |
APC
(2194035, 2194040) |
CD272
(BTLA) |
MIH26 |
Highly expressed on mature naïve and pre-germinal center cells |
|
PE
(2322525, 2322530) |
APC
(2322545, 2322550) |
CD274
(B7, PD-L1) |
29E.2A3 |
Increased expression on on pre-germinal center and unswitched B-cells |
BV421
(2248565, 2248570) |
PE
(2248525, 2248530)PE/Cy7
(2248585, 2248590) |
APC
(2147035, 2147040) |
CD275
(ICOSL, B7-H2) |
2D3 |
Expressed on pro-, pre- and immature B cells |
|
PE
(2147015, 2147020) |
APC
(2147035, 2147040) |
CD317
(BST2) |
RS38E |
General B cell marker |
|
PE
(2342025, 2342030) |
APC
(2342050)AF647
(2342015, 2342020) |
CD319
(CRACC) |
162.1 |
Expressed on pre-germinal center B cells and more abundant on unswitched memory cells |
|
PE
(2259030) |
APC
(2259045, 2259050) |
CD351
(Fcα/µ receptor) |
TX61 |
Expressed on B cells |
|
|
|
IgG Fc |
HP6017 |
B-cell maturation |
BV421
(2646585, 2646590) |
FITC
(2646545, 2646550)PerCP/Cy5.5
(2646555, 2646560) |
APC
(2646525, 2646530)AF647
(2646595, 2646600)APC/Cy7
(2646565, 2646570) |
IgM |
MHM-88 |
B-cell maturation |
PacBlue
(2172565, 2172570)BV421
(2172575, 2172580)BV510
(2172605, 2172610)
BV570
(2172585)
BV605
(2172615, 2172620)
BV650
(2172625, 2172630) |
FITC
(2172530)PerCP/Cy5.5
(2172555, 2172560)PE
(2172535, 2172540) |
APC
(2172545, 2172550)APC/Cy7
(2172595, 2172600) |
Pre-BCR |
HSL2 |
Expressed on Pro-, and early pre- B cells |
|
PE
(2339520) |
|