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Key B Cell Markers

Marker Clone Function Violet Laser Blue Laser Red Laser
CD19 HIB19 B-cell lineage marker

PacBlue
(2111115, 2111120)BV421
(2111165, 2111170)BV510
(2111205, 2111210)

BV570
(2111175, 2111180)

BV605
(2111215, 2111220)

BV650
(2111185, 2111190)

BV711
(2111225, 2111230)

BV785
(2111195, 2111200)

FITC
(2111025, 2111030)A488
(2111095)PerCP
(2111135, 2111140)

PerCP/Cy5.5
(2111145, 2111150)

PE
(2111035, 2111040)

PE/Dazzle 594
(2111255, 2111260)

PE/Cy5
(2111045, 2111050)

PE/Cy7
(2111075, 2111080)

APC
(2111055, 2111060)A647
(2111110, 2111100)A700
(2111125, 2111130)

APC/Cy7
(2111085, 2111090)
SJ25C1

BV521
(2415085, 2415090)BV510
(2415095, 2415100)BV605
(2415115, 2415120)

BV650
(2415125, 2415120)

BV7112415105, 2415110)

BV785
(2415135, 2415140)

FITC
(2415035, 2415040)PerCP
(2415065, 2415070)PerCP/Cy 5.5
(2415075, 2415080)

PE
(2415015, 2415020)

PE/Cy7
(2415055, 2415060)

 APC
(2415025, 2415030)APC/CY7
(2415045, 2415050)
CD20 2H7 Expressed on all B cells with levels increasing through B cell maturity

PacBlue
(2111595, 2111600)BV421
(2111645, 2111650)BV510
(2111695, 2111700)

BV570
(2111655)

BV605
(2111665, 2111670)

BV650
(2111675, 2111680)

BV711
(2111705, 2111710)

FITC
(2111515, 2111520)AF488
(2111580)PerCP
(2111615, 2111620)

PerCP/Cy5.5
(2111625, 2111630)

PE
(2111525, 2111530)

PE Dazzle 594
(2111735, 2111740)

PE/Cy5
(2111535, 2111540)

PE/Cy7
(2111555, 2111560)

APC
(2111545, 2111550)AF647
(2111590)AF700
(2111610)

APC/Cy7
(2111565, 2111570)
CD38 HB-7 B cell differentiation BV510
(2383055, 2383060)

FITC
(2383045, 2383050)PerCP/Cy5.5
(2383065, 2383070)PE
(2383015, 2383020)

PE/Cy7
(2383035, 2383040)

 APC
(2383025, 2383030)APC/ Cy7
(2383075, 2383080)
HIT2 BV421
(2117625, 2117630)BV605
(2117655, 2117660)BV711
(2117635, 2117640)

FITC
(2117515, 2117520)AF488
(2117555, 2117560)PerCP
(2117595, 2117600)

PerCP/Cy5.5
(2117605, 2117610)

PE
(2117525, 2117530)

PE Dazzle 594
(2117685, 2117690)

PE/Cy5
(2117535, 2117540)

PE/Cy7
(2117575, 2117580)

APC
(2117545, 2117550)AF647
(2117570)AF700
(2117615, 2117620)

APC/Cy7
(2117665, 2117670)
IgD IA6-2 B-Cell Differentation

PacBlue
(2341115, 2341120)BV421
(2341125, 2341130)BV510
(2341095, 2341100)

BV605
(2341155, 2341160)

 AF488
(2341075, 2341080)PerCP
(2341165, 2341170)

APC
(2341105, 2341110)AF647
(2341135, 2341140)AF700
(2341145, 2341150)

APC/ Cy7
(2341085, 2341090)

Other B Cell Markers

Marker Clone Function Violet Laser Blue Laser Red Laser
CD1d 51.1 Downregulated upon B-cell activation BV510
(2351565, 2351570)		 PerCP/Cy5.5
(2351555, 2351560)PE
(2351525, 2351530)PE/Cy7 2351545, 2351550)		 APC
(2351535, 2351540)
CD5 L17F12 Differentiation None PerCP/Cy5.5 (2420025, 2420030), PE/Cy7 (2420035, 2420040) APC/Cy7 (2420045, 2420050)
CD5 UCHT2 Differentiation PacBlue
(2103115, 2103120)BV421
(2103125, 2103130)

FITC
(2103025, 2103030)PerCP
(2103085, 2103090)PerCP/Cy5.5
(2103095, 2103100)

PE
(2103035, 2103040)

PE/Cy7
(2103105, 2103110)

 APC
(2103055, 2103060)AF647
(2103080)
CD10 HI10a Expressed prior to DJ rearrangement BV421
(2161085, 2161090)BV510
(2161095, 2161100)BV605
(2161105, 2161110)

FITC
(2161035, 2161040)PerCP/Cy5.5
(2161075, 2161080)PE
(2161015, 2161020)

PE/Cy5
(2161030)

PE/Cy7
(2161065, 2161070)

 APC
(2161045, 2161050)APC/Cy7
(2161060)
CD20
(cytoplasmic)		 1412 Useful for intracellular staining of CD20 AF488
(2302520)PerCP/Cy5.5
(2302540)PE
(2302550)		 AF647
(2302530)
CD21 Bu32 Important for complement binding

FITC
(2374545, 2374550)PerCP/Cy5.5
(2374535, 2374540)PE
(2374515, 2374520)

PE/Cy7
(2374555, 2374560)

 APC
(2374525, 2374530)
CD23 EBVCS-5 Regulates the production of IgE BV421
(2292605, 2292610)

FITC
(2292525, 2292530)PerCP/Cy5.5
(2292585, 2292590)PE
(2292535, 2292540)

PE/Cy7
(2292580)

 APC
(2292565, 2292570)APC/Cy7
(2292600)
CD24 ML5 Decreased expression with B cell activation; not expressed on plasma cells BV5421
(2155605, 2155610)BV510
(2155625, 2155630)BV605
(2155615, 2155620)

FITC
(2155515, 2155520)AF488
(2155540)PerCP
(2155565, 2155570)

PerCP/Cy5.5
(2155575, 2155580)

PE
(2155525, 2155530)

PE/Cy7 2155595, 2155600)

 APC
(2155585, 2155590)AF647
(2155545, 2155550)
CD27 LG.3A10 Memory and activation marker PacBlue
(1221085, 1221090)		 PerCP/Cy5.5
(1221065, 1221070)PE/Cy7
(1221075, 1221080)		 APC
(1221055, 1221060)
M-T271 PacBlue
(2382065, 2382070)BV421
(2382085, 2382090)BV510
(2382095, 2382100)

FITC
(2382015, 2382020)PerCP/Cy5.5
(2382035, 2382040)PE
(2382025, 2382030)

PE Dazzle 594
(2382105, 2382110)

PE/Cy7
(2382055, 2382060)

 APC
(2382045, 2382050)AF700
(2382075, 2382080)
O323

PacBlue
(2114105, 2114110)BV421
(2114115, 2114120)BV510
(2114175, 2114180)

BV570
(2114125)

BV605
(2114145, 2114150)

BV650
(2114135, 2114140)

BV711
(2114165, 2114170)

BV785
(2114155, 2114160)

FITC
(2114025, 2114030)PerCP
(2114085, 2114090) PerCP/Cy5.5
(2114095, 2114100)PE
(2114035, 2114040)

PE/Cy7
(2114185, 2114190

APC
(2114045, 2114050)AF647
(2114060)AF700
(2114065, 2114070)

APC/Cy7
(2114075, 2114080)
CD48 BJ40 Highly expressed on all B cell subsets PacBlue
(2283510)		 FITC
(2283525, 2283530)PE
(2283535, 2283540)
CD70 113-16 Expressed on activated B cells; involved in expansion and differentiation FITC
(2375525, 2375530)PerCP/Cy 5.5
(2375535, 2375540)PE
(2375515, 2375520)		 APC
(2375545, 2375550)
CD79a HM47 Important for B cell development and function PerCP/Cy 5.5
(2267540)PE
(2267515, 2267520)PE/Cy7
(2267545, 2267450)		 APC
(2267525, 2267530)
CD80 2D10 Expressed on germinal center cells BV421
(2126105, 2126110)BV605
(2126125)BV650
(2126135)

FITC
(2126025, 2126030)AF488
(2126065, 2126070)PE
(2126035, 2126040)

PE/Cy5
(2126045, 2126050)

PE/Cy7
(2126085, 2126090)
CD81
(TAPA-1)		 5A6 Important for B-cell signaling

FITC
(2347515, 2347520)PerCP/Cy5.5
(2347535, 2347540)PE
(2347525, 2347530)

PE/Cy7
(2347555, 2347560)

 APC
(2347545, 2347550)
CD84 CD84.1.21 Highly expressed on all B cell subsets PE
(2230035, 2230040)
CD93 VIMD2 B-lineage precursor molecule PE
(2280535, 2280540)
CD126 UV4 Expressed on plasmablasts and plasma cells PerCP/Cy5.5
(2364055, 2364060)PE
(2364015, 2364020)PE/Cy7
(2364045, 2364050)		 APC
(2364025, 2364030)
CD138
(Syndecan-1)		 DL-101 Plasma cell marker FITC
(2361515, 2361520)PerCP/Cy5.5
(2361545, 2361550)PE
(2361525, 2361530)		 APC
(2361535, 2361540)
Ml15

BV421
(2382575, 2382580)BV510
(2382585, 2382590)BV605
(2382595, 2382600)

BV711
(2382605, 2382610)

FITC
(2382535, 2382540)PerCP/Cy5.5
( 2382545, 2382550)PE
(2382515, 2382520)

PE/Cy7
(2382565, 2382570)

 APC
(2382525, 2382530)AF647
(2382615, 2382620)AF700
(2382555, 2382560)
CD150
(SLAM)		 A12
(7D4)		 Expressed on B cell subsets FITC
(2131530)AF488
(2131560)PE
(2131535, 2131540)
CD156c
(ADAM10)		 SHM14 May be involved in regulation of IgE PE
(2363515, 2363520)		 APC
(2363525, 2363530)
CD170
(Siglec-5)		 1A5 Expressed on a subset of mature naïve and pre-germinal center cells PE
(2360015, 2360020)		 APC
(2360025, 2360030)
CD184
(CXCR4)		 12G5 Expressed on all B cells with highest amounts on germinal center B cells BV421
(2132585, 2132590)BV605
(2132605, 2132610)

PerCP/Cy5.5
(2132575, 2132580)PE
(2132525, 2132530)PE Dazzle 594
(2132625, 2132630)

PE/Cy5
(2132535, 2132540)

PE/Cy7
(2132565, 2132570)

 APC
(2132545, 2132550)
CD185
(CXCR5)		 J252D4 Expressed on all B cells PacBlue
(2384585, 2384590)BV5421
(2384595, 2384600)BV510
(2384605, 2384610)

FITC
(2384565, 2384570)AF488
(2384555, 2384560)PerCP/Cy5.5
(2384545, 2384550)

PE
(2384515, 2384520)

PE Dazzle 594
(2384635, 2384640)

PE/Cy7
(2384615, 2384620)

APC
( 2384535, 2384540)AF647
(2384525, 2384530)AF700
(2384575, 2384580)

APC/Cy7
(2384625, 2384630)
CD196
(CCR6)		 GO34E3 Expressed on pre-germinal center B cells

BV521
(2367035, 2367040)BV510
(2367115)BV605
(2367095, 2367100)

BV650
(2367125, 2367130)

BV785
(2367105, 2367110)

FITC
(2367055, 2367060)AF488
(2367065, 2367070)PerCP/Cy5.5
(2367025, 2367030)

PE
(2367045, 2367050)

PE Dazzle 594
(2367145, 2367150)

PE/Cy7
(2367085, 2367090)

 APC
(2367075, 2367080)AF647
(2367015, 2367020)
CD252
(OX40L, TNFSF4)		 11C3.1 Expressed at all stages of B-cell development PE
(2231535, 2231540)
CD255
(TWEAK)		 CARL-1 Differentially expressed on B-cell subsets PE
(2141525)
CD267
(TACI, TNFRSF13B)		 1A1 Has a negative regulatory role in B cell homeostasis PE
(2159530)PE Dazzle 594
(2159545, 2159550)
CD268
(BAFF-R, TNFR)		 11C1 Important for B cell development and survival

FITC
(2184520)PerCP/Cy5.5
(2184585, 2184590)PE
(2184525, 2184530)

PE/Cy7
(2184595, 2184600)

 APC
(2184580)AF647
(2184570)APC/Cy7
(2184560)
CD269
(BCMA, TNFRSF17)		 19F2 Important for plasma cell survival. Upregulated upon activation. PE
(2387515, 2387520)		 APC
(2387525, 2387530)
CD270
(HVEM, TR2)		 122 Differentially expressed on B cell subsets PerCP/Cy5.5
(2194055, 2194060)PE
(2194025, 2194030)PE/Cy7
(2194045, 2194050)		 APC
(2194035, 2194040)
CD272
(BTLA)		 MIH26 Highly expressed on mature naïve and pre-germinal center cells PE
(2322525, 2322530)		 APC
(2322545, 2322550)
CD274
(B7, PD-L1)		 29E.2A3 Increased expression on on pre-germinal center and unswitched B-cells BV421
(2248565, 2248570)		 PE
(2248525, 2248530)PE/Cy7
(2248585, 2248590)		 APC
(2147035, 2147040)
CD275
(ICOSL, B7-H2)		 2D3 Expressed on pro-, pre- and immature B cells PE
(2147015, 2147020)		 APC
(2147035, 2147040)
CD317
(BST2)		 RS38E General B cell marker PE
(2342025, 2342030)		 APC
(2342050)AF647
(2342015, 2342020)
CD319
(CRACC)		 162.1 Expressed on pre-germinal center B cells and more abundant on unswitched memory cells PE
(2259030)		 APC
(2259045, 2259050)
CD351
(Fcα/µ receptor)		 TX61 Expressed on B cells
IgG Fc HP6017 B-cell maturation BV421
(2646585, 2646590)		 FITC
(2646545, 2646550)PerCP/Cy5.5
(2646555, 2646560)		 APC
(2646525, 2646530)AF647
(2646595, 2646600)APC/Cy7
(2646565, 2646570)
IgM MHM-88 B-cell maturation

PacBlue
(2172565, 2172570)BV421
(2172575, 2172580)BV510
(2172605, 2172610)

BV570
(2172585)

BV605
(2172615, 2172620)

BV650
(2172625, 2172630)

 FITC
(2172530)PerCP/Cy5.5
(2172555, 2172560)PE
(2172535, 2172540)		 APC
(2172545, 2172550)APC/Cy7
(2172595, 2172600)
Pre-BCR HSL2 Expressed on Pro-, and early pre- B cells PE
(2339520)

B-Cell Differentiation:

Specificity Clone Fluorochrome Purpose Cat. No. Name
CD19 HIB19 PE B-cell lineage marker 2111035 PE Anti-Human CD19
CD27 O323 FITC Memory and activation marker 2114025 FITC Anti-Human CD27
CD38 HIT2 APC B-cell differentiation 2117545 APC Anti-Human CD38
CD20 2H7 PE/Dazzleâ„¢ 594 Levels increase with B-cell maturity 2111735 PE/Dazzleâ„¢ 594 Anti-Human CD20
CD24 ML5 PerCP-Cy5.5 Decreased expression with B cell activation; not expressed on plasma cells 2155575 PerCP-Cy5.5 Anti-Human CD24

B Memory Panel:

Specificity Clone Fluorochrome Purpose Cat. No. Name
IgD IA6-2 Alexa Fluor® 488 Differentiation 2341075 Alexa Fluor® 488 Anti-human IgD Antibody
CXCR3 IC6/CXCR3 PE Homing 2368525 PE anti-human CD183
(CXCR3)
CD24 ML5 Alexa Fluor® 647 Differentiation, epithelial adhesion 2155545 Alexa Fluor® 647 anti-human CD24
CD21 Bu32 PE/Cy7 Activation, BCR co-receptor for antigen-bound C3d 2374555 PE/Cy7 anti-human CD21
CD38 HB-7 PerCP-Cy5.5 Differentiation 2383065 PerCP/Cy5.5 anti-human CD38
CD45/B220 RA3/6B2 PE-Cy5 Differentiation 1116045 PE/Cy5 anti-mouse/human CD45R/B220
CD3 OKT3 Brilliant Violet 711â„¢ Exclusion 2186635 Brilliant Violet 711â„¢ anti-human CD3
CD27 M-T271 Brilliant Violet 421â„¢ Differentiation, receptor for CD70 on activated T cells 2382085 Brilliant Violet 421â„¢ anti-human CD27
CD95 DX2 PE/Dazzleâ„¢ 594 Activation; pro-apoptotic in the presence of activated CD95L T cells 2128165 PE/Dazzleâ„¢ 594 anti-human CD95
(Fas)
CD19 HIB19 Brilliant Violet 605â„¢ Lineage 2111215 Brilliant Violet 605â„¢ anti-human CD19

Panel 2 Based On OMIP-003

"Wei C, Jung J, Sanz I, OMIP-003: Phenotypic Analysis of Human Memory B Cells: Cytometry A. 2011 November; 79

(11): 894-896."