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With literally thousands of commercially available fluorochrome conjugated antibodies for flow cytometry, there are many choices for your application. In many cases several clones are available to the same target. How do you evaluate which clones are best for your research?
Define your application– Are you using live or fixed samples? Will you be looking at intracellular antigens such as cytokines or tran...
Spectral flow cytometry has been a part of the Sony Biotechnology portfolio since 2013, and the ID7000™ Spectral Cell Analyzer is the latest addition to our range. This innovative system enables user-friendly multiparametric detection with a number of key benefits, including improved flexibility in panel design, the ability to re-use spectral references, and tools for removal of autofluorescence. Below are some key areas where this spectral...
Flow cytometry has long been considered a tool of hematologists and immunologists who primarily work with the hematopoetic system. Flow cytometry is capable of performing the simultaneous detection of more than 20 parameters; however it requires the sample to be prepared into a single cell suspension. The need for a single cell suspension makes the sample preparation more intensive for cells derived from tissues. Many tissues are also high...
Presented by Fluoro-Finder
In this webinar, we will explore the key considerations when designing multicolor panels for use on the ID7000™ spectral cell analyzer. We will review the available fluorochromes with respect to their spectral profiles and highlight how similaritie...
Much of cell research happens in the context of cultured or modified cells. More biologically relevant experiments can be done with blood. However, blood cannot entirely capture what takes place within a live organism. In their paper Futamura, et al. describes a method to study the movement and subsequent interactions of immune cells in live mice by flow cytometry.
To study cell movement, KikGR knock-in mice were us...
1. Purchase larger sizes of common antibodies on established fluorochromes. Packaging and shipping antibodies can be costly. Therefore smaller sizes typically cost greater than two times more compared to larger sizes.
2. Titrate your antibodies for optimal performance in your assay. In the majority of cases you will use a lot less antibody and have better results.
3. Properly ...