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Recent advances in genome editing and the application of fluorescent proteins have accelerated Interest in isolating specific populations of brain cells from mixed populations. Researchers are also using cell sorting to isolate single cells for expansion and analysis.
Follow these 5 guidelines to address the challenge of obtaining single cell suspensions from delicate brain tissues.
The methods chosen t...
Human Stem Cell Markers
Embryonic
Surface
Embryonic
Marker
Clone
Purpose/Comment...
Cell sorting is a valuable technique for isolating cells from mixed populations. This technique can be challenging when working with tissues such as brain, as it requires single cell suspensions. With recent advances in genome editing and fluorescent protein applications, the interest in sorting brain cells has been increasing. Several studies have been published in which scientists have successfully isolated stem cells from whole brain hom...
T-cells are part of the adaptive immune system with several subtypes including CD8+ effector T-cells and CD4+ helper T-cells. Main categories can be divided into several sub-categories. What are the critical markers to study T-cells? From the key subsets how can other subsets be defined?
Simplest panel
This panel identifies the main T-cell populations. These markers are expressed at high level...
Key Extracellular Treg Markers
Key Extracellular Treg Markers
Marker
Clone
Purpose
Violet Laser
protocols
We’ve prepared this section as a guide to help you prepare various cell types for analysis using flow cytometry. Use it as a starting point and expect that it will require further optimization for your investigations.
Cell Preparation of Tissue Culture Cells
Flow Cytometry Reagents for Cancer Research – Beyond Annexin V
The Annexin V assay which measures exposed phosphatidyl serines, a early marker of apoptosis, is a well established assay for cancer research. Using flow cytometry, apoptotic cells can be distinguished from dead cells, with a semi-quantitiative read-out. With the ability to measure multiple parameters simulataneously, flow cytometry is ...
The KIRAVIA Dyes™ are organic polymers with fluorescent dyes loaded precisely o...