Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride.

Formulation

References

1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
5. Boldin MP, et al. 2011. J Exp Med. PubMed
6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
15. Han W, 2013. J. Immunol. 190:4786. PubMed
16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
25. Ma Y, et al. 2014. Cancer Res. 74:436.