Purified anti-rat IFN-γ

Antibodies Single
Sony
DB-1
Other
Mouse IgG1, κ
Mouse,Rat
Recombinant rat IFN-γ
3139010
$279.00

Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-γ). The DB-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The DB-1 antibody has been well characterized for ELISPOT, ELISA, intracellular staining, Western blotting, IHC, and neutralization (in vitro and in vivo).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, the suggested use of this reagent is 1-4 ug/ml. To obtain a linear standard curve, serial dilutions of IFN-γ recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. The purified DB-1 has been tested by blocking fluorochrome conjugated DB-1 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Van der Meide P, et al. 1989. Lymphokine Res. 8:439.
2. Nennesmo I, et al. 1989. Brain Res. 504:306.
3. Rayner D, et al. 1987. Scand. J. Immunol. 25:621.
4. Hartung H, et al. 1990. Ann Neurol. 27:247.
5. Bernard I, et al. 1998. Eur. Cytokine Net. 9:613.