Biotin anti-rat CD54
Description
CD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.References
1. Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC)
2. Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP)
3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
4. Willenborg DO, et al. 1996. J. Immunol. 157:1973.
5. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)