Description

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on activated T and B cells, monocytes, hepatocytes, and plasma cells. High affinity IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity, but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself, but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. The D7715A7 (15A7) antibody blocks IL-6/IL-6 receptor interactions.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP)
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5. Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP)
6. Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block)
7. Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed
8. Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed
9. Sintes J, et al. 2010. J. Leukoc. Biol. 88:687.  PubMed