Description

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
5. Xiao L, et al. 2014. J Endod. 41:479. PubMed