Description

CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 microg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
3. Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)