Purified anti-human CD100

Antibodies Single
Sony
A8
Other
Mouse IgG1, κ
Human
Human PHA activated lymphocytes
25 µg
2242005
$61.00

Description

CD100, a 150 kD homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Hall K, et al. 1996. P. Natl. Acad. Sci. USA 93:11780.
2. Mizrahi S, et al. 2007. PLoS One. 2(9):e818.
3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)