PE anti-human CD170 (Siglec-5)
Description
CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Cornish AL, et al. 1998. Blood 92:2123.
2. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
3. Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
4. Angata T, et al. 2006. FASEB J. 20:1964.
5. Avril T, et al. 2005. J. Biol. Chem. 280:19843.