PE anti-human CD14

Antibodies Single
Sony
M5E2
Flow Cytometry
Mouse IgG2a, κ
Human
Full-length human CD14 protein
2109250
$139.00

Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Formulation

microg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the microg size, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
5. Williams KC, et al. 2001. J. Exp. Med. 193:905. (IF)
6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Sriuswan S, et al. 2014. PLoS One. 9:110321. PubMed
10. Fisher JP et al. 2014. Clin Cancer Res. 20:5720. PubMed