Pacific Blue™ anti-human CD45

Antibodies Single
Sony
HI30
Flow Cytometry
Mouse IgG1, κ
Human
2120105
$121.00

Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
microg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 microL per 106 cells in 100 microL volume.
For microg sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 microg per 106 cells in 100 microL volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Kishihara K, et al. 1993. Cell 74:143.
3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
5. Nagano M, et al. 2007. Blood 110:151.
6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed