Pacific Blue™ anti-human CD31

Antibodies Single
Sony
WM59
Flow Cytometry
Mouse IgG1, κ
Human
2115565

Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 microg per 106 cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
10. Santoro SP, et al. 2015. Cancer Immunol Res. 3:68. Pubmed