Pacific Blue™ anti-human CD279 (PD-1)

Antibodies Single
Sony
EH12.2H7
Flow Cytometry
Mouse IgG1, κ
Human
2249580
$318.00

Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0microg per 106 cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

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4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)