Pacific Blue™ anti-human CD19

Antibodies Single
Sony
HIB19
Flow Cytometry
Mouse IgG1, κ
Human
2111120
$260.00

Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
microg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test sizes, the suggested use of this reagent for immunofluorescent staining is 5 microL per 106 cells in 100 microL volume. 
For microg sizes, the suggested use of this reagent for immunofluorescent staining is ≤ 0.5 microg per 106 cells in 100 microL volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
9. Santos JM, et al. 2013. J Transl Med. 11:18. PubMed
10. Bartholomaeus P, et al. 2014. J. Immunol. 192:2091. PubMed
11. Steinsbo O, et al. 2014. Nat Commun. 5:4041. PubMed
12. Della-Torre E, et al. 2014. Ann Rheum Dis. PubMed
13. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed