Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 microg per 10⁶ cells in 100 microL volume or 100 microL of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
6. Kuchen S, et al. 2007. J. Immunol. 179:5886.
7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed
8. Schoenbrunn A, et al. 2012 J. Immunol. 189:5985 PubMed.