Pacific Blue™ anti-human CD14

Antibodies Single
Sony
M5E2
Flow Cytometry
Mouse IgG2a, κ
Human
Full-length human CD14 protein
2109140
$278.00

Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
microg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 microL per million cells or 5 microL per 100 microL of whole blood.
For microg sizes, the suggested use of this reagent is ≤ 2.0 microg per 106 cells in 100 microL volume or 100 microL of whole blood.
It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
5. Williams KC, et al. 2001. J. Exp. Med. 193:905. (IF)
6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9. Tungatt K, et al. 2015. J Immunol. 194:463. PubMed
10. Li H, et al. 2015. J Infect Dis. 211:1717. PubMed