Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α₃ domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

References

1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
3. Evans RL, et al. 1981. Immunol. 78:544.
4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
6. Carbone A, et al. 1999. Blood 93:2319. (IHC)
7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)