Brilliant Violet 510™ anti-human CD3

Antibodies Single
Sony
UCHT1
Flow Cytometry
Mouse IgG1, κ
Human
2102240
$434.00

Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

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3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
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10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed.