Biotin anti-human CD69

Antibodies Single
Sony
FN50
Immunofluorescence
Mouse IgG1, κ
Human
100 µg
2154620
$105.00

Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 microg per 106 cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)