APC/Cy7 anti-human CD69

Antibodies Single
Sony
FN50
Flow Cytometry
Mouse IgG1, κ
Human
2154570
$306.00

Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
8. Tsai CY, et al. 2015. J Immunol. 194:3890. PubMed