Alexa Fluor® 700 anti-human CD34
Description
CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
References
1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
2. Felschow DM, et al. 2001. Blood 97:3768.
3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
7. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
8. Breton G, et al. 2015. J Exp Med. 212:401. PubMed