Alexa Fluor® 647 anti-human CD80

Antibodies Single
Sony
2D10
Flow Cytometry
Mouse IgG1, κ
Human
2126080
$260.00

Description

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

References

1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed