Alexa Fluor® 647 anti-human CD317 (BST2, Tetherin)
Description
CD317, also known as BST2, Tetherin, and HM1.24, is a type II transmembrane GPI-protein with a molecular weight of about 29-33 kD. It is an interferon-induced protein expressed on dendritic cells, plasma cells, B lymphoblast cells, monocytes, granulocytes, T cells, NK cells, stromal cells, and some non-hematopoietic cells. BST2 inhibits cytokine production through interaction with ILT7 (CD85g). It is also involved in the regulation of B cell growth. More importantly, BST2 has been found to restrict the release of a number of viruses from infected cells, including all tested retroviruses (such as HIV-1) and some arenaviruses and filoviruses. In HIV-1 studies, it has been reported that BST2 retains the nascent virons on the surface of infected cells by incorporation of the protein into HIV-1 particles. HIV-1 Vpu is able to induce BST2 degradation.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.