Alexa Fluor® 647 anti-human CD147

Antibodies Single
Sony
HIM6
Flow Cytometry
Mouse IgG1, κ
Human
Human PBMCs
2131050
$260.00

Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. For immunofluorescence microscopy, a concentration range of 5-10 μg per ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 microg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

References

1. Menashi S, et al. 2003. Cancer Res. 63:7575. (WB, IHC)
2. Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
3. Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
4. Porat-Shliom N, et al. 2013. PLoS One. 25:81897. PubMed