Alexa Fluor® 488 anti-human CD95 (Fas)

Antibodies Single
Sony
DX2
Flow Cytometry
Mouse IgG1, κ
Human
CD95 transfected L cells
2128080
$290.00

Description

CD95 is a 45 kD single chain type I glycoprotein also known as Fas, APO-1, and TNFRSF6. It is a member of the TNF receptor superfamily. CD95 is expressed on T and B lymphocytes, monocytes, neutrophils, and fibroblasts. CD95 expression is upregulated by activation. The extracellular region of CD95 binds to CD178 (Fas ligand). CD178 binding to CD95 induces apoptosis and has been shown to play a role in the maintenance of peripheral tolerance.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Schlossman S, et al. Eds.1995. Leucocyte Typing V. Oxford University Press. New York.
2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
3. Cifone M, et al. 1994. J. Exp. Med. 180:1547. (Apop)
4. Zietz C, et al. 2001. Am. J. Pathol. 159:963. (IHC)
5. Sergi C, et al. 2000. Am. J. Pathol. 156:1589. (IHC)
6. Xie S, et al. 2010. J. Immunol. 184:2289. (FC)  PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
8. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)