Alexa Fluor® 488 anti-human CD86

Antibodies Single
Sony
IT2.2
Flow Cytometry
Mouse IgG2b, κ
Human
2127065
$121.00

Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)