Alexa Fluor® 488 anti-human CD11a

Antibodies Single
Sony
HI111
Flow Cytometry
Mouse IgG1, κ
Human
100 tests
2106080
$260.00

Description

CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Leite F, et al. 2002. Infec. Immun. 70:4336.
3. Jiang Y, et al. 2005. Clin. Hemorheol. Microcircul. 32:261.
4. Béchard D, et al. 2001. J. Immunol. 167:3099.
5. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
6. Choi EY, et al. 2008. Blood 111:3607. PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
8. Ma Q, et al. 2002. J. Biol. Chem. 277:10638.