-
Sign In
- Sony Biotechnology
- Sony Biotechnology
SLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being tranlocated to the plasma membrane and facilitating the assembly of a multi-subunit complex comprised of Vav1, Nck, ITK, and PLCγ1. SLP76 also plays an essential role in normal T cell development.
SLP76 also represents a negative regulatory node in the TCR activation pathway. Following TCR engagement, SLP76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase (HPK1). This modification promotes an interaction between SLP76 and the scaffolding protein 14-3-3ε, resulting in dissociation of the TCR signaling complex and a diminution of TCR signaling.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.