Tag-it Violet™ Proliferation and Cell Tracking Dye
Description
Tag-it Violet™ Proliferation and Cell Tracking Dye can be used for cell tracking and for proliferation assays. Tag-it Violet™ passively diffuses into the cell where esterases cleave acetoxymethyl esters (AM) on the molecule, Tag-it Violet™ then covalently attaches to intracellular proteins enabling its long-term retention.
Tag-it Violet™ is excited with a fluorescence wavelength of 395 nm, and emits at 455 nm. Cells labeled wtih Tag-it Violet™ can be analyzed by flow cytometry in instruments equipped with a violet laser, or visualized by fluorescence microscopy using a DAPI filter.
Recommended Usage
This lot has been tested by flow cytometry for in vitro cell proliferation. It can be used at concentrations ranging from 1 - 20 µM for cell labeling. It is recommended that the reagent be titrated for optimal performance for each cell type, culturing condition, or application.