PE/Dazzle™ 594 anti-mouse/human Helios

Antibodies Single
Sony
22F6
Flow Cytometry
Hamster IgG
Human,Mouse
Helios peptide (aa 51-107)
1286160
$467.00

Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry . For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

References

1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed