Brilliant Violet 510™ anti-mouse H-2Kd

Antibodies Single
Sony
SF1-1.1
Flow Cytometry
Mouse IgG2a, κ
Mouse
BALB/c Mouse cells
50 µg
1183125
$283.00

Description

The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.

References

1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
2. Abasto JP, et al. 1993. J. Immunol. 151:3569. (WB)
3. Bashuda H, et al. 1997. Transplantation 63:113.
4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
8. Delon J, et al. 1998. Immunity 9:467.