Brilliant Violet 421™ anti-mouse H-2Kd

Antibodies Single
Sony
SF1-1.1
Flow Cytometry
Mouse IgG2a, κ
Mouse
BALB/c Mouse cells
50 µg
1183115
$278.00

Description

The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

References

1. Noun G, et al. 1996. J. Immunol. 157:2455. (IP)
2. Abasto JP, et al. 1993. J. Immunol. 151:3569. (WB)
3. Bashuda H, et al. 1997. Transplantation 63:113.
4. Sester M, et al. 2000. J. Biol. Chem. 34:113. (IP)
5. Ma XT, et al. 2006. Cancer Res. 66:1169. (FC)
6. Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
7. Norian L, et al. 2004. J. Immunol.. 173:835. PubMed
8. Delon J, et al. 1998. Immunity 9:467.