APC/Cy7 anti-mouse CD45.1

Antibodies Single
Sony
A20
Flow Cytometry
Mouse IgG2a, κ
Mouse
SJL mouse thymocytes and splenocytes
1153575
$116.00

Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
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3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
4. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC)
6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC)
7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC)
8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
9. Mende I, et al. 2006. Blood 107:1383. (IF, IHC, FC)
10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
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16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
20. Burgess MR, et al. 2014. Blood. 124:3947. PubMed