Description

IL-2 is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells, promoting proliferation and maturation. Additionally, IL-2 has been found to stimulate growth and differentiation of B cells, NK cells, LAK cells, monocytes, and oligodendrocytes.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 10⁶ cells in 100 microL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
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3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
8. Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
9. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
10. Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
12. Rout N, et al. 2010. PLoS One 5:e9787. (FC)