Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The B27 antibody reacts with the human interferon-γ. The B27 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Favre C, et al. 1989. Molec. Immunol. 26:17. (Neut)
2. Kaur A, et al. 2002. J Virol. 76:3646.
3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (Neut)
4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
5. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
6. Acosta-Rodriguez EV, et al. 2007. Nat. Immunol. 9:942-9. (Neut)
7. Gangur V, et al. 1998. FASEB J. 12:705-13. (Neut)