Description

Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 1-2 microg per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF)
4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
5. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
6. Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed