Description

Ly49G is a type II transmembrane protein belonging to the family of Ly49 proteins, each having specificity for a small repertoire of MHC I alleles that are generally recognized in a peptide-independent manner.   The Ly49 family of MHC class I receptors shows a high degree of variability between mouse strains.  It is expressed on a subset of NK cells and some NKT cell lines.  The AT8 antibody reacts with the Ly49G receptor from BALB/c, 129/J, 129Sv/J, SJL, FVB/N, A/J, C3H/HeJ, C3H/HeNCr, CBA/J, and CBA/NCr mice.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 microg per 10⁶ cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Makrigiannis AP, et al. 2004.  J. Immunol.  172:1414.