Purified anti-mouse Ly-6C

Antibodies Single
Sony
HK1.4
Flow Cytometry,Immunohistochemistry
Rat IgG2c, κ
Mouse
L3 cloned CTL cells
1240010
$242.00

Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
2. Herold KC, et al. 1990. Diabetes  39:815. (Activ)
3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed