Purified anti-mouse I-Ad
Description
The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Hiramine C, et al. 1995. Cell. Immunol. 160:157.
2. Wang Z, et al. 2004. J. Immunol. 172:5924.
3. Ma XT, et al. 2006. Cancer Research 66:1169.
4. Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed
5. Tian C, et al. 2007. J. Immunol. 179:6762.